Abstract:
: A simple, specific, sensitive and rapid reverse phase high performance liquid chromatographic (HPLC) method for
the determination of racecadotril (RCD) was developed and validated. Sample preparation involved simple dissolution, followed
by dilution with mobile phase to eliminate any chromatographic solvent effects. RCD was quantitated on a C18 column (4.6 mm
i.d. × 300 mm length), using a mobile phase composed of acetonitrile: water (65:35 %v/v) which was delivered at a flow rate of
1.0 mL/min. The method was proven to be linear over a concentration range of 5 to 100 μg/mL with a mean correlation
coefficient of 0.9991. The intra-day and inter-day precision (coefficient of variation) were in the range of 0.53% to 0.92% and
0.95% to 1.76%, respectively. The intra-day accuracy (relative error) were in the range of 2.53% to 4.47% and the inter-day
accuracy were in the range of 3.11% to 4.92%. The limit of detection (LOD) and the limit of quantification (LOQ) of the
developed method were determined to be 0.5 μg/mL and 5 μg/mL, respectively. The developed method was established as a
rapid analytical tool as it required short retention time, high precision, sensitivity and small volumes of sample for analysis
