Abstract:
The manuscript describes validated High-Performance Thin-Layer Chromatography (HPTLC) method for the estimation of Nebivolol (NEB) and Amlodipine
(AML) in combined dosage form. The HPTLC separation was achieved on an aluminium-backed layer of silica gel 60F254 using chloroform-methanol-toluene-ammonia (8+ 1.8+ 1+ 0.3, v/v/v/v/) as mobile phase. Quantitation was achieved with UV detection at 273 nm over the concentration range 400-1000 ng/spot and
200 – 800 ng/spot for Nebivolol and Amlodipine, respectively with mean recovery of 100.3 ± 0.76 and 100.5 ± 0.92 % for Nebivolol and Amlodipine by HPTLC
method. These method were found to be Simple, sensitive, accurate, precise, reproducible, and economical can be applicable for the simultaneous determination
of Nebivolol and Amlodipine in combined dosage form.
Description:
The authors are greatly thankful to Torrent Pharmaceutical Ltd, Indrad, India
for providing gift sample of NEB and AML and S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Kherva for providing all
the facilities to carry out the work.
