Abstract:
Plant tissue culture is a method used to grow and propagate plants outside of their native habitat in a controlled laboratory setting. The purpose of the current investigation was to clarify the potential function of bacterial auxin in plant tissue culture. In order to achieve this, MS basal medium was supplemented with bacterial supernatant containing auxin, and the effect of this addition on in vitro plant growth was investigated. With internodal explants inoculated on MS + BS, callus induction was observed at the apex of explant at 1 and 2 ml, where adventitious root induction is observed In ND1, ND2 and ND-3. In treatment plant ND3 shows significant increase of carbohydrate and root length with number of advantage root number was increase as compared to other ND1. In comparison of control plant no one is increase root num or length, were not reported increase of in terms of biomass carbohydrates and protein.
