Abstract:
Plant tissue culture is a method used to grow and propagate plants outside of their native habitat in a
controlled laboratory setting. The purpose of the current investigation was to clarify the potential
function of bacterial auxin in plant tissue culture. In order to achieve this, MS basal medium was
supplemented with bacterial supernatant containing auxin, and the effect of this addition on in vitro
plant growth was investigated. With internodal explants inoculated on MS + BS, callus induction was
observed at the apex of explant at 1 and 2 ml, where adventitious root induction is observed In DS1, DS2
and DS-3. In treatment plant DS3 shows significant increase of carbohydrate and root length with
number of advantage root number was increase as compared to other DS1. In comparison of control
plant no one is increase root num or length, were not reported increase of in terms of biomass
carbohydrates and protein.
