STUDIES ON THE OPTIMIZATION OF LIPASE PRODUCTION BY Aspergillus parasiticus MK178553 ISOLATED FROM THE PADDY FIELD OF SOIL

Abstract

Extracellular lipase producer fungal stain was screened in the laboratory into paddy soil sample collected from Achhoti Village, Kumhari, near Bhilai Region, Chhattisgarh. Identification was done through 18S rRNA as Aspergillus parasiticus MK178553. In present investigation the maximum lipase production in shake flask by using submerged fermentation from basal medium. The work has been studied to evaluate the effect of different physical process factors like incubation period, pH, temperature, agitation, and substrate as inducer on lipase production. In mineral Growth Medium by using submerged fermentation showed 4.217 U/ml of lipase activity after 96 h of fermentation at 4 days of incubation period in highly basic pH 8.0 on 150rpm rotation by using shake flask method