Abstract:
Clindamycin is a potent lincosamide antibiotic against susceptible anaerobic
bacteria and gram-positive aerobes. Furthermore, CLN also has anti-inflammatory
activities. It is applied topically in the management of acne vulgaris.The current
research was focused on gel loaded for the topical delivery to Clindamycin
phosphate to overcome short half life problem of the drug and to minimize the side
effects of drug with conventional gel. The FTIR study revealed that there was no
interaction between drug and polymer and the combination is suitable for
preparation of microsponges. Clindamycin phosphate loaded microsponges were
prepared by oil in oil emulsion solvent diffusion method as the drug is highly water
soluble and belongs to BCS class III. The microsponges were prepared by using
ethyl cellulose as the polymer and incorporated into carbopol gel. The
microsponges were evaluated for visual inspection, % yield, % drug content, %
entrapement efficiency and particle size. The effect of formulation variables like
stirring speed and drug:polymer ratio were evaluated on microsponges. The
optimized formulation with drug:polymer ratio of 1:1, stirring speed of 1000rpm
had the drug content of 78.69% and percent entrapement efficiency was found to
be 87.94% The results concluded that as the drug:polymer ratio increases, drug
content and entrapement efficiency decreases. The particle size of optimized batch
of microsponges was found to be 37.44μm. The results of particle size analysis
concluded that as the drug:polymer ratio increases, particle size increases. The
microsponges of batch CLPA1-CLPA9 were subjected to in-vito drug release
study by Franz diffusion cell apparatus. The batch showed the controlled release of
95.49% of drug release at 10 hrs. The results of in-vitro drug release showed that
as the drug:polymer ratio increases, more controlled release formulation is
obtained. The microsponges were incorporated into carbopol gel was evaluated for visual inspection, pH, spreadability and stability of gel at room temperature for 1
month. The transparent white microsponge gel had the pH of 6.38 with
spreadability of 7.05 g cm/s. The gel was found to be stable after 1 month. In-vitro
antibacterial activity was observed for microsponge gel and compared with
marketed 1%w/w CLP gel (Clindac A).
