DSpace Repository

Isolation, characterization and chromatography based purification of antibacterial compound isolated from rare endophytic actinomycetes micrococcus yunnanensis

Show simple item record

dc.contributor.author Kumar, Ravi Ranjan
dc.contributor.author Jadeja, Vasantba
dc.date.accessioned 2023-05-08T05:50:09Z
dc.date.available 2023-05-08T05:50:09Z
dc.date.issued 2017
dc.identifier.citation Ranjan, R., & Jadeja, V. (2017). Isolation, characterization and chromatography based purification of antibacterial compound isolated from rare endophytic actinomycetes Micrococcus yunnanensis. Journal of pharmaceutical analysis, 7(5), 343-347. en_US
dc.identifier.issn 2095-1779
dc.identifier.uri http://10.9.150.37:8080/dspace//handle/atmiyauni/877
dc.description This work was supported by United Grant Commission, Pune, India (Grant no. 47-1328/10) for financial support. The authors are thankful to management of Shree M. & N. Virani Science College, Rajkot, India, for providing necessary research facilities en_US
dc.description.abstract Endophytic actinomycetes are considered as one of the relatively unexplored potential sources in search of antibiotic producer against antibiotic resistant pathogens. A potent strain isolated from Catharanthus roseus that displays antibacterial potential against antibiotic resistant human pathogen Staphylococcus aureus was characterized and designated as Micrococcus yunnanensis strain rsk5. Rsk5 is capable of producing optimum antibacterial metabolites on starch casein medium at 30 °C, pH 5 and 2% NaCl condition. The crude antibacterial agent was extracted from fermentation broth by ethyl acetate and separated by TLC using chloroform-methanol (24:1, v/v) solvent system with Rf value of 0.26. It was partially purified by flash chromatography, followed by HPLC and analyzed by ultraviolet visible spectrophotometer to get absorption maxima at 208.4 nm. The ESI-MS spectra showed molecular ion peaks at m/z 472.4 [M-H], which does not match with any known antibacterial compound. en_US
dc.description.sponsorship UGC,Pune en_US
dc.language.iso en en_US
dc.publisher Journal of Pharmaceutical Analysis en_US
dc.subject Micrococcus yunnanensis strain rsk5 en_US
dc.subject Chromatograph en_US
dc.subject Antibacteria en_US
dc.subject Endophytes en_US
dc.subject Catharanthus roseus en_US
dc.title Isolation, characterization and chromatography based purification of antibacterial compound isolated from rare endophytic actinomycetes micrococcus yunnanensis en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account