Abstract:
Thermophilic lipases are stable at higher temperatures, which enhance
their demand in industrial applications. In the present study,
thermostable lipase was produced from bacterial strain Bacillus
subtilis SHVSC04 (MN565992) isolated from Tuva Timba hot spring,
Gujarat, India. Isolate displayed maximum growth in basal medium
augmented with 3% sucrose, 3% yeast extract and 2 % salt at pH 7 and
50°C in 48 h. Whereas, isolate produced maximum lipase in tributyrin
agar medium with pH 7, enriched with 3% sucrose, 3% yeast extract
and incubated at 50°C in 72 h. Tributyrin (2%) was found to be the best
substrate for lipase production in submerged conditions. The amount of
lipase was increased by 1.5 fold upon optimization of different
environmental and nutritional parameters. The enzyme retained 84%
activity at 60°C and 70% of activity at 70°C for 1h. The present
findings advocate that hot springs in Gujarat are a substantial source of
thermostable bacteria producing enzymes of industrial importance.
