DSpace Repository

Optimization of process parameters for enhanced thermostable lipase production by bacillus subtilis SHVSC04

Show simple item record

dc.contributor.author Bhatt, Shweta
dc.contributor.author Pandhi, Neepa
dc.date.accessioned 2023-05-10T03:46:32Z
dc.date.available 2023-05-10T03:46:32Z
dc.date.issued 2019-11
dc.identifier.citation Ambasana,L. & Pandhi,N.(2019).Optimization of process parameters for enhanced thermostable lipase production by bacillus subtilis SHVSC04.International Journal of Advanced Research,7(11),211-221.http://dx.doi.org/10.21474/IJAR01/9995 en_US
dc.identifier.issn 2320-5407
dc.identifier.uri http://10.9.150.37:8080/dspace//handle/atmiyauni/908
dc.description.abstract Thermophilic lipases are stable at higher temperatures, which enhance their demand in industrial applications. In the present study, thermostable lipase was produced from bacterial strain Bacillus subtilis SHVSC04 (MN565992) isolated from Tuva Timba hot spring, Gujarat, India. Isolate displayed maximum growth in basal medium augmented with 3% sucrose, 3% yeast extract and 2 % salt at pH 7 and 50°C in 48 h. Whereas, isolate produced maximum lipase in tributyrin agar medium with pH 7, enriched with 3% sucrose, 3% yeast extract and incubated at 50°C in 72 h. Tributyrin (2%) was found to be the best substrate for lipase production in submerged conditions. The amount of lipase was increased by 1.5 fold upon optimization of different environmental and nutritional parameters. The enzyme retained 84% activity at 60°C and 70% of activity at 70°C for 1h. The present findings advocate that hot springs in Gujarat are a substantial source of thermostable bacteria producing enzymes of industrial importance. en_US
dc.language.iso en en_US
dc.publisher International Journal of Advanced Research en_US
dc.title Optimization of process parameters for enhanced thermostable lipase production by bacillus subtilis SHVSC04 en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account